Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli
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چکیده
منابع مشابه
Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes.
PFGE is a valuable tool for assessing Listeria monocytogenes strain interrelatedness. It is based on the study of total bacterial DNA restriction patterns. Cells are embedded in agarose plugs before being lysed. The released DNA is then digested into large fragments by restriction enzymes. As DNA fragments are too large to be separated by traditional electrophoresis in an agarose gel, changes i...
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Viruses are the most abundant microorganisms in marine and freshwater environments and perhaps the most genetically diverse (Fuhrman and Suttle, 1993). Counting viruses in aquatic samples is now a routine matter, but assessing the diversity and dynamics within complex assemblages is still a challenge. DNA-based fingerprinting approaches, which rely on amplification of rRNA gene fragments by PCR...
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Pulsed field gel electrophoresis allows separation and visualization of very large DNA molecules. This powerful technique is predominantly used in many aspects in molecular biology and medical diagnostics. Since the procedure for the preparation of the samples involves several steps, many different parameters can be changed and subsequently influence the resolution of the results. Therefore, in...
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DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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There are approximately 1.4 million Salmonella infections annually in the US. PulseNet USA detects multi-state and regional clusters and outbreaks by the use of pulsefield gel electrophoresis (PFGE) of foodborne pathogens, and assists in the investigation of potential common source outbreaks. On July 14, 2004, PA Department of Health noted a cluster of Salmonella Javiana cases in PA involving f...
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ژورنال
عنوان ژورنال: World Journal of Microbiology & Biotechnology
سال: 2004
ISSN: 0959-3993,1573-0972
DOI: 10.1007/s11274-004-1004-3